Journal: EMBO Reports

Article Title: Muskelin is a substrate adaptor of the highly regulated Drosophila embryonic CTLH E3 ligase

doi: 10.1038/s44319-025-00397-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-eIF4E (Boster) was used at 1:10000.

Techniques: SYBR Green Assay, Imaging, Recombinant, Sequencing, Software, Microscopy, Ion-Mobility Spectrometry, Mass Spectrometry

Journal: Cancer gene therapy

Article Title: Targeting and killing of prostate cancer cells using lentiviral constructs containing a sequence recognized by translation factor eIF4E and a prostate-specific promoter.

doi: 10.1038/sj.cgt.7700885

Figure Lengend Snippet: Figure 3 Western analysis of eIF4E level in cancer and noncancer cell lines. Protein extracts from cancer cells (LNCaP, MCF-7, PC- 3M, and DU145) and noncancer cells (BPH-1, Plat-E, Huvec-c, and 267-B1) were probed with antibodies to eIF4E and b-actin in a Western blot. Density scans (means7s.e.m., n ¼ 3) of eIF4E/b-actin are shown in the bottom panel.

Article Snippet: The membranes were blocked with 5% skim milk in trisbuffered saline (TBS) containing 0.05% tween-20 and probed with 1:1000 rabbit polyclonal anti-eIF4E antibody (Cell Signalling), 1:500 anti-HTK (a gift from Dr William C Summers, Yale University), or 1:500 anti-b-actin antibody (Sigma), followed by 1:2000 polyclonal goat anti-rabbit antibody conjugated to horseradish peroxidase (Bio-Rad).

Techniques: Western Blot